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Culture Method Quiz

1. At which temperature does Saccharomyces kudriavzevii grow best?  *This question is required.
2. How much time does Staphylococcus aureus need to grow? *This question is required.
3. Which Pseudomonas strain requires these conditions for growth?

Media: Tryptic Soy Agar (Soybean Casein Digest Agar), Nonselective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar

Temperature: 25°C

Atmosphere: Aerobic

Growth Time: 24 – 48 hours
  *This question is required.
4. Which atmosphere does Campylobacter jejuni require to grow? *This question is required.
5. If using a candle jar to grow Neisseria gonorrhoeae, how long should you wait before opening the inoculated agar medium petri plate? *This question is required.
6. Clostridium perfringens grows best on which agar? *This question is required.
7. Which strain requires these conditions for growth? 

Media
Phase 1: MRS (Man, Rogosa, Sharpe) Broth
Phase 2: Columbia CNA with Sheep Blood or Tryptic Soy Agar with Sheep Blood

Temperature
Phase 1: 35°C
Phase 2: 35°C

Atmosphere
Phase 1: Aerobic
Phase 2: 5% to 7% Carbon dioxide

Growth Time
Phase 1: 48 hours
Phase 2: 48 hours *This question is required.
8. How much time does Trichophyton spp. need to grow? *This question is required.
9. Which strain requires microscopic examination to visualize the colonies? *This question is required.
10. On which two agars does Mycobacterium tuberculosis grow best? *This question is required.
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